Nawab Ali, PhD
Research Associate Professor
Academic interests include biochemistry and biotechnology, cell and molecular, and membrane biology. Research interests are directed towards understanding receptor mediated signal transduction as well as intracellular signaling mechanisms in relation to environmental changes or in disease states. These projects involve studies on the role of G-proteins and their receptors, second messengers such as inositol polyphosphates, cyclic nucleotides (cAMP, cGMP), protein kinases and phosphatases in regulation of cellular processes. Among inositol polyphosphate second messengers, we are particularly interested in higher inositol polyphosphates i.e. inositol polyphosphate pyrophosphates (IP7 and IP8) in relation to their metabolism, cellular levels under adverse conditions and their role in cellular processes. Emphasis is placed on subcellular compartmentalization, vesicular membrane trafficking, protein-protein interactions, and cellular regulation of apoptosis, endo- and exocytosis, and maintenance of cell polarityin epithelial cells. My laboratory is also interested in bioinformatics studying global profiling of gene expression using microarray and proteomics approaches under altered environmental conditions. We are particularly interested in identification of functional motives and their phylogenetic adaptations by higher life forms. Biomedical engineering interests include development and characterization of biocompatible materials for medical implants and computational modeling of carcinogenesis.
- Regulation of inositol polyphosphate metabolites and their role in mediating cellular processes
- Influence of microgravity on cellular signaling and gene expression
- Molecular cloning and characterization of G-protein coupled receptors
- Biocompatible polymers for endothelialization of cardiovascular stents
Current Lab Members
- Rakhee Agarwal, M.S. PhD Candidate, Applied Bioscience Graduate Program
- Aparna Kunidala, M.S. MS Student, Biology Graduate Program
- Zakir Hossain PhD Student, Applied Bioscience Program
- Samar Hassen PhD Student, Applied Bioscience Program
- Recombinant DNA Methods and Applications (ASCI 7386)
- Biomaterials Science and Applications (ASCI 7399)
- Biochemistry of Biological Molecules (ASCI 7375)
- Microbiology (BIOL 2401)
- De, S., Sharma, S., Trigwell, S., Laska, B., Ali, N., Mazumder, M. K. and Mehta, J. L. Endothelial Cell Growth Studies on Plasma-Treated Polyurethane Coatings for a Long Term Biocompatibility. Journal of Biomaterials Science, Polymer Edition., 2004 (in press)
- Ahangar, R., Ali, N., Iqbal K. and Altmayer, K. Biodynamic Modeling and Simulation of Multistage Cell Mutations. DNA and Cell Biology, 23 (10), 625-633, 2004.
- Cornett, L.E., Kirby, J.D., Vizcara, J.A., Ellison, J.F., Thrash, J., Mayeux, P.R., Crew, M.D., Jones, S.M., ALI, N. and Baeyens, D.A.: Molecular cloning and functional characterization of a vasotocin receptor subtype expressed in the pituitary gland of the domestic chicken (Gallus domesticus): Avian homolog of the mammalian V1b-vasopressin receptor. Regul. Pept. 110, 231-291, 2003.
- Mazumder, M.M., De, S., Trigwell, S., Ali, N, Mazumder, M.K. and Mehta, J.L. Corrosion resistance of polyurethane-coated Nitinol cardiovascular stents. Corrosion resistance of polyurethane-coated Nitinol cardiovascular stents. Journal of Biomaterials Science, Polymer Edition, 14(12), 1351-1362, 2003.
- Wyse B. D., ALI, N. and Ellison, D. H.: Interaction with grp58 increases activity of the thiazide sensitive Na-Cl cotransporter. Am. J. Physiol. Renal Physiol., 282, F424-30, 2002.
- ALI, N., Yousufzai, S.Y.K. and Abdel-Latiff, A.A.: Activation of particulate guanylate cyclase by adrenomedullin in cultured SV-40 transformed cat iris sphincter smooth muscle (SV-CISM-2) cells. Cell Signal. 12, 491-498, 2000.
- Yousufzai, S.Y.K., ALI, N. and Abdel-Latif, A.A.: Effects of adrenomedullin on cyclic AMP formation and on relaxation in iris sphincter smooth muscle. Invest. Ophthalmol. Vis. Sci. 40, 3245-3253, 1999.
- Ding, K-H., ALI, N. and Abdel-Latif, A.A.: Atrial natriuretic peptide provokes a dramatic increase in cyclic GMP formation and markedly inhibits muscarinic-stimulated Ca 2+ mobilization in SV-40 transformed cat sphincter smooth muscle (SV-CISM-2) cells.Cell. Signal. 11, 87-94, 1999.
- ALI, N., Kantachuvesiri, S., Smallwood, J.S., Macala, L.J., Isabelle, C. and Hayslett, J.P.: Vasopressin induced activation of protein kinase C in renal epithelial cells. Biochim. Biophys. Acta, 1402, 188-196, 1998.
- ALI, N., Macala, L.J. and Hayslett, J.P.: Identification and characterization of MARCKS in Xenopus laevis. Biochem. Biophys. Res. Commun., 234, 143-146, 1997.
- ALI, N., Craxton, A., Sumner, M. and Shears, S. B.: Effects of aluminum on the hepatic inositol polyphosphate phosphatase. Biochem. J. 305, 557-561, 1995.
- Craxton, A., ALI, N. and Shears, S.B.: Comparison of the activities of a multiple inositol polyphosphate phosphatase obtained from several sources: a search for heterogeneity in this enzyme. Biochem. J. 305, 491-498, 1995.
- Ye, W., ALI, N., Bembenek, M. E., Shears, S. B. and Lafer, E. M.: Inhibition of clathrin assembly by high- affinity binding of specific inositol polyphosphates to the synapse specific clathrin assembly protein AP-3. J. Biol. Chem. 270, 1564-1568, 1995.
- Shears, S.B., ALI, N., Craxton, A. and Bembenek, M.E.: Synthesis and metabolism of bis- diphosphoinositol tetrakisphosphate in vitro and in vivo. J. Biol. Chem. 270, 10489-10497, 1995.
- ALI, N., Duden, R., Bembenek, M.E. and Shears S.B.: The interaction of coatomer with inositol polyphosphates is conserved in Saccharomyces cerevisiae. Biochem. J., 310, 279-284, 1995.
- ALI, N. and Agrawal, D. K.: Guanine nucleotide binding regulatory proteins: their characteristics and identification (a review article). J. Pharmacol. Toxicol. Methods 32: 187-196, 1994.
- ALI, N., Craxton, A. and Shears, S. B.: Hepatic Ins(1,3,4,5)P4 3-phosphatase is compartmentalized inside endoplasmic reticulum. J. Biol. Chem. 268(9): 6161-6167, 1993.
- ALI, N., Aligue, R., and Evans, W. H.: Highly purified bile canalicular vesicles and lateral plasma membranes isolated from rat liver on Nycodenz gradients: Biochemical and immunolocalization studies. Biochem. J. 271: 185-192, 1990.
- ALI, N., and Evans, W. H.: Priority targeting of glycosylphosphatidylinositol anchored proteins to the bile canalicular (apical) plasma membrane of hepatocytes: Involvement of "late" endosomes. Biochem. J. 271: 193-199, 1990.
- ALI, N., and Evans, W. H.: Distribution of polypeptide binding guanosine-5'[35S)thio]_trisphosphate and anti-(ras-protein) antibodies in liver subcellular fractions. Biochem. J. 271: 179-183, 1990.
- ALI, N., Milligan, G., and Evans, W. H.: Distribution of G_proteins in rat liver plasma membrane domains and endocytic pathways. Biochem. J. 261: 906-912, 1989.
- American Society for Biochemistry and Molecular Biology
- American Association for the Advancement of Science